Comparative osteogenesis of maxilla and iliac crest human bone marrow stromal cells attached to oxidized titanium - a pilot study
Identifieur interne : 002132 ( Pmc/Curation ); précédent : 002131; suivant : 002133Comparative osteogenesis of maxilla and iliac crest human bone marrow stromal cells attached to oxidized titanium - a pilot study
Auteurs : Sunday O. Akintoye [États-Unis] ; Parascevi Giavis ; Derek Stefanik [États-Unis] ; Lawrence Levin [États-Unis] ; Francis K. Mante [États-Unis]Source :
- Clinical oral implants research [ 0905-7161 ] ; 2008.
Abstract
Severe alveolar bone loss affects dental implant placement. Bone augmentation by grafting iliac crest bone rich in osteoprogenitor cells like bone marrow stromal cells (BMSCs) requires a second surgical procedure in non-orofacial bone. Skeletal site-specific osteogenesis indicates maxilla and mandible BMSCs are highly proliferative and exhibit osteogenic properties superior to iliac crest BMSCs. Alveolar bone can be easily obtained during routine dental surgery, but it is unclear if titanium-attached alveolar BMSCs will retain their superior osteogenic properties. This study evaluated and compared
Primary culture of maxilla and iliac crest BMSCs from four normal healthy volunteers were expanded in culture. In 24-well plates, first passage BMSCs were seeded directly (1 × 104 cells/well) on oxidized titanium discs (1.27cm diameter and 2mm thickness) or tissue culture plate. Each cell type was assessed for affinity for titanium, post-attachment survival and osteogenic differentiation based on alkaline phosphatase and osteopontin expressions.
There was no difference in the affinity of maxilla and iliac crest BMSCs to titanium. However, titanium-attached maxilla BMSCs were apparently more osteogenically responsive than iliac crest cells based on calcium accumulation and gene expression of alkaline phosphatase and osteopontin. But these differences were not statistically significant in this small patient sample.
Maxilla and iliac crest BMSCs have similar attachment affinity for titanium. This pilot study indicate that titanium-attached maxilla BMSCs were more osteogenically responsive and may be a viable and more readily available donor graft material in implant dentistry.
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DOI: 10.1111/j.1600-0501.2008.01592.x
PubMed: 18983324
PubMed Central: 2905681
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Parascevi Giavis<affiliation><nlm:aff id="A4"> Ursinus College, Collegeville PA</nlm:aff><wicri:noCountry code="subfield">Collegeville PA</wicri:noCountry></affiliation>Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Comparative osteogenesis of maxilla and iliac crest human bone marrow stromal cells attached to oxidized titanium - a pilot study</title><author><name sortKey="Akintoye, Sunday O" sort="Akintoye, Sunday O" uniqKey="Akintoye S" first="Sunday O" last="Akintoye">Sunday O. Akintoye</name><affiliation wicri:level="2"><nlm:aff id="A1"> Department of Oral Medicine, School of Dental Medicine, University of Pennsylvania, Philadelphia, PA</nlm:aff><country xml:lang="fr">États-Unis</country><placeName><region type="state">Pennsylvanie</region></placeName><wicri:cityArea> Department of Oral Medicine, School of Dental Medicine, University of Pennsylvania, Philadelphia</wicri:cityArea></affiliation></author><author><name sortKey="Giavis, Parascevi" sort="Giavis, Parascevi" uniqKey="Giavis P" first="Parascevi" last="Giavis">Parascevi Giavis</name><affiliation><nlm:aff id="A4"> Ursinus College, Collegeville PA</nlm:aff><wicri:noCountry code="subfield">Collegeville PA</wicri:noCountry></affiliation></author><author><name sortKey="Stefanik, Derek" sort="Stefanik, Derek" uniqKey="Stefanik D" first="Derek" last="Stefanik">Derek Stefanik</name><affiliation wicri:level="2"><nlm:aff id="A1"> Department of Oral Medicine, School of Dental Medicine, University of Pennsylvania, Philadelphia, PA</nlm:aff><country xml:lang="fr">États-Unis</country><placeName><region type="state">Pennsylvanie</region></placeName><wicri:cityArea> Department of Oral Medicine, School of Dental Medicine, University of Pennsylvania, Philadelphia</wicri:cityArea></affiliation></author><author><name sortKey="Levin, Lawrence" sort="Levin, Lawrence" uniqKey="Levin L" first="Lawrence" last="Levin">Lawrence Levin</name><affiliation wicri:level="2"><nlm:aff id="A2"> Department of Oral and Maxillofacial Surgery, School of Dental Medicine, University of Pennsylvania, Philadelphia, PA</nlm:aff><country xml:lang="fr">États-Unis</country><placeName><region type="state">Pennsylvanie</region></placeName><wicri:cityArea> Department of Oral and Maxillofacial Surgery, School of Dental Medicine, University of Pennsylvania, Philadelphia</wicri:cityArea></affiliation></author><author><name sortKey="Mante, Francis K" sort="Mante, Francis K" uniqKey="Mante F" first="Francis K" last="Mante">Francis K. Mante</name><affiliation wicri:level="2"><nlm:aff id="A3"> Department of Preventive and Restorative Sciences, School of Dental Medicine, University of Pennsylvania, Philadelphia, PA</nlm:aff><country xml:lang="fr">États-Unis</country><placeName><region type="state">Pennsylvanie</region></placeName><wicri:cityArea> Department of Preventive and Restorative Sciences, School of Dental Medicine, University of Pennsylvania, Philadelphia</wicri:cityArea></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">18983324</idno><idno type="pmc">2905681</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2905681</idno><idno type="RBID">PMC:2905681</idno><idno type="doi">10.1111/j.1600-0501.2008.01592.x</idno><date when="2008">2008</date><idno type="wicri:Area/Pmc/Corpus">002132</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">002132</idno><idno type="wicri:Area/Pmc/Curation">002132</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">002132</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Comparative osteogenesis of maxilla and iliac crest human bone marrow stromal cells attached to oxidized titanium - a pilot study</title><author><name sortKey="Akintoye, Sunday O" sort="Akintoye, Sunday O" uniqKey="Akintoye S" first="Sunday O" last="Akintoye">Sunday O. Akintoye</name><affiliation wicri:level="2"><nlm:aff id="A1"> Department of Oral Medicine, School of Dental Medicine, University of Pennsylvania, Philadelphia, PA</nlm:aff><country xml:lang="fr">États-Unis</country><placeName><region type="state">Pennsylvanie</region></placeName><wicri:cityArea> Department of Oral Medicine, School of Dental Medicine, University of Pennsylvania, Philadelphia</wicri:cityArea></affiliation></author><author><name sortKey="Giavis, Parascevi" sort="Giavis, Parascevi" uniqKey="Giavis P" first="Parascevi" last="Giavis">Parascevi Giavis</name><affiliation><nlm:aff id="A4"> Ursinus College, Collegeville PA</nlm:aff><wicri:noCountry code="subfield">Collegeville PA</wicri:noCountry></affiliation></author><author><name sortKey="Stefanik, Derek" sort="Stefanik, Derek" uniqKey="Stefanik D" first="Derek" last="Stefanik">Derek Stefanik</name><affiliation wicri:level="2"><nlm:aff id="A1"> Department of Oral Medicine, School of Dental Medicine, University of Pennsylvania, Philadelphia, PA</nlm:aff><country xml:lang="fr">États-Unis</country><placeName><region type="state">Pennsylvanie</region></placeName><wicri:cityArea> Department of Oral Medicine, School of Dental Medicine, University of Pennsylvania, Philadelphia</wicri:cityArea></affiliation></author><author><name sortKey="Levin, Lawrence" sort="Levin, Lawrence" uniqKey="Levin L" first="Lawrence" last="Levin">Lawrence Levin</name><affiliation wicri:level="2"><nlm:aff id="A2"> Department of Oral and Maxillofacial Surgery, School of Dental Medicine, University of Pennsylvania, Philadelphia, PA</nlm:aff><country xml:lang="fr">États-Unis</country><placeName><region type="state">Pennsylvanie</region></placeName><wicri:cityArea> Department of Oral and Maxillofacial Surgery, School of Dental Medicine, University of Pennsylvania, Philadelphia</wicri:cityArea></affiliation></author><author><name sortKey="Mante, Francis K" sort="Mante, Francis K" uniqKey="Mante F" first="Francis K" last="Mante">Francis K. Mante</name><affiliation wicri:level="2"><nlm:aff id="A3"> Department of Preventive and Restorative Sciences, School of Dental Medicine, University of Pennsylvania, Philadelphia, PA</nlm:aff><country xml:lang="fr">États-Unis</country><placeName><region type="state">Pennsylvanie</region></placeName><wicri:cityArea> Department of Preventive and Restorative Sciences, School of Dental Medicine, University of Pennsylvania, Philadelphia</wicri:cityArea></affiliation></author></analytic><series><title level="j">Clinical oral implants research</title><idno type="ISSN">0905-7161</idno><idno type="eISSN">1600-0501</idno><imprint><date when="2008">2008</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><sec id="S1"><title>Objectives</title><p id="P1">Severe alveolar bone loss affects dental implant placement. Bone augmentation by grafting iliac crest bone rich in osteoprogenitor cells like bone marrow stromal cells (BMSCs) requires a second surgical procedure in non-orofacial bone. Skeletal site-specific osteogenesis indicates maxilla and mandible BMSCs are highly proliferative and exhibit osteogenic properties superior to iliac crest BMSCs. Alveolar bone can be easily obtained during routine dental surgery, but it is unclear if titanium-attached alveolar BMSCs will retain their superior osteogenic properties. This study evaluated and compared <italic>in vitro</italic> osteogenic properties of titanium-attached maxilla and iliac crest BMSCs in same individuals.</p></sec><sec sec-type="materials|methods" id="S2"><title>Materials and Methods</title><p id="P2">Primary culture of maxilla and iliac crest BMSCs from four normal healthy volunteers were expanded in culture. In 24-well plates, first passage BMSCs were seeded directly (1 × 10<sup>4</sup> cells/well) on oxidized titanium discs (1.27cm diameter and 2mm thickness) or tissue culture plate. Each cell type was assessed for affinity for titanium, post-attachment survival and osteogenic differentiation based on alkaline phosphatase and osteopontin expressions.</p></sec><sec id="S3"><title>Results</title><p id="P3">There was no difference in the affinity of maxilla and iliac crest BMSCs to titanium. However, titanium-attached maxilla BMSCs were apparently more osteogenically responsive than iliac crest cells based on calcium accumulation and gene expression of alkaline phosphatase and osteopontin. But these differences were not statistically significant in this small patient sample.</p></sec><sec id="S4"><title>Conclusion</title><p id="P4">Maxilla and iliac crest BMSCs have similar attachment affinity for titanium. This pilot study indicate that titanium-attached maxilla BMSCs were more osteogenically responsive and may be a viable and more readily available donor graft material in implant dentistry.</p></sec></div></front></TEI><pmc article-type="research-article" xml:lang="EN"><pmc-comment>The publisher of this article does not allow downloading of the full text in XML form.</pmc-comment>
<pmc-dir>properties manuscript</pmc-dir>
<front><journal-meta><journal-id journal-id-type="nlm-journal-id">9105713</journal-id><journal-id journal-id-type="pubmed-jr-id">1142</journal-id><journal-id journal-id-type="nlm-ta">Clin Oral Implants Res</journal-id><journal-title>Clinical oral implants research</journal-title><issn pub-type="ppub">0905-7161</issn><issn pub-type="epub">1600-0501</issn></journal-meta><article-meta><article-id pub-id-type="pmid">18983324</article-id><article-id pub-id-type="pmc">2905681</article-id><article-id pub-id-type="doi">10.1111/j.1600-0501.2008.01592.x</article-id><article-id pub-id-type="manuscript">NIHMS212388</article-id><article-categories><subj-group subj-group-type="heading"><subject>Article</subject></subj-group></article-categories><title-group><article-title>Comparative osteogenesis of maxilla and iliac crest human bone marrow stromal cells attached to oxidized titanium - a pilot study</article-title></title-group><contrib-group><contrib contrib-type="author"><name><surname>Akintoye</surname><given-names>Sunday O</given-names></name><xref rid="A1" ref-type="aff">1</xref></contrib><contrib contrib-type="author"><name><surname>Giavis</surname><given-names>Parascevi</given-names></name><xref rid="A4" ref-type="aff">4</xref></contrib><contrib contrib-type="author"><name><surname>Stefanik</surname><given-names>Derek</given-names></name><xref rid="A1" ref-type="aff">1</xref></contrib><contrib contrib-type="author"><name><surname>Levin</surname><given-names>Lawrence</given-names></name><xref rid="A2" ref-type="aff">2</xref></contrib><contrib contrib-type="author"><name><surname>Mante</surname><given-names>Francis K</given-names></name><xref rid="A3" ref-type="aff">3</xref></contrib></contrib-group><aff id="A1"><label>1</label> Department of Oral Medicine, School of Dental Medicine, University of Pennsylvania, Philadelphia, PA</aff><aff id="A2"><label>2</label> Department of Oral and Maxillofacial Surgery, School of Dental Medicine, University of Pennsylvania, Philadelphia, PA</aff><aff id="A3"><label>3</label> Department of Preventive and Restorative Sciences, School of Dental Medicine, University of Pennsylvania, Philadelphia, PA</aff><aff id="A4"><label>4</label> Ursinus College, Collegeville PA</aff><author-notes><corresp id="FN1">Corresponding Author: Sunday O. Akintoye BDS, DDS, MS, University of Pennsylvania, School of Dental Medicine, Department of Oral Medicine, The Robert Schattner Center Room 209, 240 South 40th Street, Philadelphia PA 19104, Phone: 215-898-9932, Fax: 215-573-7853, <email>akintoye@dental.upenn.edu</email></corresp></author-notes><pub-date pub-type="nihms-submitted"><day>14</day><month>6</month><year>2010</year></pub-date><pub-date pub-type="ppub"><month>11</month><year>2008</year></pub-date><pub-date pub-type="pmc-release"><day>19</day><month>7</month><year>2010</year></pub-date><volume>19</volume><issue>11</issue><fpage>1197</fpage><lpage>1201</lpage><abstract><sec id="S1"><title>Objectives</title><p id="P1">Severe alveolar bone loss affects dental implant placement. Bone augmentation by grafting iliac crest bone rich in osteoprogenitor cells like bone marrow stromal cells (BMSCs) requires a second surgical procedure in non-orofacial bone. Skeletal site-specific osteogenesis indicates maxilla and mandible BMSCs are highly proliferative and exhibit osteogenic properties superior to iliac crest BMSCs. Alveolar bone can be easily obtained during routine dental surgery, but it is unclear if titanium-attached alveolar BMSCs will retain their superior osteogenic properties. This study evaluated and compared <italic>in vitro</italic> osteogenic properties of titanium-attached maxilla and iliac crest BMSCs in same individuals.</p></sec><sec sec-type="materials|methods" id="S2"><title>Materials and Methods</title><p id="P2">Primary culture of maxilla and iliac crest BMSCs from four normal healthy volunteers were expanded in culture. In 24-well plates, first passage BMSCs were seeded directly (1 × 10<sup>4</sup> cells/well) on oxidized titanium discs (1.27cm diameter and 2mm thickness) or tissue culture plate. Each cell type was assessed for affinity for titanium, post-attachment survival and osteogenic differentiation based on alkaline phosphatase and osteopontin expressions.</p></sec><sec id="S3"><title>Results</title><p id="P3">There was no difference in the affinity of maxilla and iliac crest BMSCs to titanium. However, titanium-attached maxilla BMSCs were apparently more osteogenically responsive than iliac crest cells based on calcium accumulation and gene expression of alkaline phosphatase and osteopontin. But these differences were not statistically significant in this small patient sample.</p></sec><sec id="S4"><title>Conclusion</title><p id="P4">Maxilla and iliac crest BMSCs have similar attachment affinity for titanium. This pilot study indicate that titanium-attached maxilla BMSCs were more osteogenically responsive and may be a viable and more readily available donor graft material in implant dentistry.</p></sec></abstract><contract-num rid="CA1">K08 CA120875-01
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